Validating microarray data tammin sursok dating

Such RNA is generally processed through a multiple steps procedure to generate amplified amino allyl RNA (AA-a RNA) coupled with fluorescent dyes.

To compare the effectiveness of q PCR from total RNA and AA-a RNA, we used samples from the 15 donors for which we had both AA-a RNA and remaining total RNA.However, care should be taken when designing such validation experiments.Here, we report our experience with microarray validation by q PCR on AA-a RNA and we present an optimized protocol that improves the reliability of this validation.This fastidious protocol introduces supplementary bias in the microarray technique, sometimes leading to false positive discovery and erroneous results [ technology (Nu GEN, San Carlos, CA, USA).Optimization of the amplification procedure has been tackled by previous investigators, such as Waddell et al.